Analytical, Diagnostic and Therapeutic Techniques, and Equipment

Enzyme-Linked Immunosorbent Assay

An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.

National Library of MedicineMedical Subject Headings2026

Structured Summary

Abstract

An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.

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Synonyms

7 entry terms
  • ELISA
  • Assay, Enzyme-Linked Immunosorbent
  • Assays, Enzyme-Linked Immunosorbent
  • Enzyme Linked Immunosorbent Assay
  • Enzyme-Linked Immunosorbent Assays
  • Immunosorbent Assay, Enzyme-Linked
  • Immunosorbent Assays, Enzyme-Linked

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Aspects Covered

14 allowable subheadings

Indexed with the subheadings adverse effects, classification, economics, ethics, history, instrumentation, methods, mortality, nursing, psychology, standards, statistics & numerical data, trends, veterinary.

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Indexing Annotation

NIM; IMMUNOSORBENTS is also available

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History Note

86(77); was see under IMMUNOENZYME TECHNIQUES 1977-85

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Previous Indexing

  • Immunoassay (1966-1976)
  • Serodiagnosis (1966-1976)

MeSH Hierarchy

Tree Numbers

  1. E05.478.566w.350.170w
  2. E05.478.566w.380w.360w
  3. E05.478.583w.400.170w
  4. E05.601.470w.350.170w
  5. E05.601.470w.380w.360w

AMA Style

References

  1. National Library of Medicine. Enzyme-Linked Immunosorbent Assay. Medical Subject Headings (MeSH). 2026. Unique ID D004797. http://id.nlm.nih.gov/mesh/2026/D004797
  2. Enzyme-Linked Immunosorbent Assay. In: Wikipedia. https://en.wikipedia.org/wiki/ELISA
  3. Enzyme-Linked Immunosorbent Assay. In: Wikidata. https://www.wikidata.org/wiki/Q332126