Analytical, Diagnostic and Therapeutic Techniques, and Equipment

Enzyme Assays

Methods used to measure the relative activity of a specific enzyme or its concentration in solution. Typically an enzyme substrate is added to a buffer solution containing enzyme and the rate of conversion of substrate to product is measured under controlled conditions. Many classical enzymatic assay methods involve the use of synthetic colorimetric substrates and measuring the reaction rates using a spectrophotometer.

National Library of MedicineMedical Subject Headings2026

Structured Summary

Abstract

Methods used to measure the relative activity of a specific enzyme or its concentration in solution. Typically an enzyme substrate is added to a buffer solution containing enzyme and the rate of conversion of substrate to product is measured under controlled conditions. Many classical enzymatic assay methods involve the use of synthetic colorimetric substrates and measuring the reaction rates using a spectrophotometer.

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Synonyms

19 entry terms
  • Enzymatic Assays
  • Assay, Enzymatic
  • Assay, Enzyme
  • Assays, Enzymatic
  • Assays, Enzyme
  • Enzymatic Assay
  • Enzyme Assay
  • Indirect Enzymatic Assays
  • Indirect Enzyme Assays
  • Assay, Indirect Enzymatic
  • Assay, Indirect Enzyme
  • Assays, Indirect Enzymatic
  • Assays, Indirect Enzyme
  • Enzymatic Assay, Indirect
  • Enzymatic Assays, Indirect
  • Enzyme Assay, Indirect
  • Enzyme Assays, Indirect
  • Indirect Enzymatic Assay
  • Indirect Enzyme Assay

MeSH Record

Aspects Covered

10 allowable subheadings

Indexed with the subheadings classification, economics, ethics, history, instrumentation, methods, standards, statistics & numerical data, trends, veterinary.

MeSH Record

History Note

2010

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AMA Style

References

  1. National Library of Medicine. Enzyme Assays. Medical Subject Headings (MeSH). 2026. Unique ID D057075. http://id.nlm.nih.gov/mesh/2026/D057075
  2. Enzyme Assays. In: Wikipedia. https://en.wikipedia.org/wiki/Enzyme_assay
  3. Enzyme Assays. In: Wikidata. https://www.wikidata.org/wiki/Q5381543