Chemicals and Drugs

Fluorescein-5-isothiocyanate

Fluorescent probe capable of being conjugated to tissue and proteins. It is used as a label in fluorescent antibody staining procedures as well as protein- and amino acid-binding techniques.

National Library of MedicineMedical Subject Headings2026

Structured Summary

Abstract

Fluorescent probe capable of being conjugated to tissue and proteins. It is used as a label in fluorescent antibody staining procedures as well as protein- and amino acid-binding techniques.

Chemical Identity

Chemical Information

Molecular Formula
C21H11NO5S
Molecular Weight
389.4 g/mol
CAS Registry No.
3326-32-7
IUPAC Name
3',6'-dihydroxy-6-isothiocyanatospiro[2-benzofuran-3,9'-xanthene]-1-one
InChIKey
MHMNJMPURVTYEJ-UHFFFAOYSA-N
SMILES
C1=CC2=C(C=C1N=C=S)C(=O)OC23C4=C(C=C(C=C4)O)OC5=C3C=CC(=C5)O
MeSH Unique ID
D016650
Source
PubChem CID 18730

MeSH Record

Classification

Broader headings

Related Concepts

Knowledge Graph

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Drug Class

Pharmacological Action

MeSH Record

Synonyms

8 entry terms
  • 5-Isothiocyanatofluorescein
  • FITC
  • 5 Isothiocyanatofluorescein
  • Fluorescein 5 isothiocyanate
  • Fluorescein (5 or 6)-Isothiocyanate
  • Fluorescein-5-isothiocyanate Hydrochloride
  • Fluorescein 5 isothiocyanate Hydrochloride
  • Hydrochloride, Fluorescein-5-isothiocyanate

MeSH Record

Aspects Covered

19 allowable subheadings

Indexed with the subheadings administration & dosage, adverse effects, analogs & derivatives, analysis, chemical synthesis, chemistry, classification, economics, history, isolation & purification, metabolism, pharmacokinetics, pharmacology, poisoning, radiation effects, standards, supply & distribution, therapeutic use, toxicity.

MeSH Record

History Note

92; was FLUORESCEIN-5-ISOTHIOCYANATE (NM) 1978-91

MeSH Record

Previous Indexing

  • Fluoresceins (1966-1991)
  • Thiocyanates (1966-1991)

MeSH Hierarchy

Tree Numbers

  1. D02w.455w.426w.779w.347.400
  2. D02w.500.375w.250
  3. D02w.886w.250w.250
  4. D03w.633.300.953.275.400
  5. D04w.711w.347.400

AMA Style

References

  1. National Library of Medicine. Fluorescein-5-isothiocyanate. Medical Subject Headings (MeSH). 2026. Unique ID D016650. http://id.nlm.nih.gov/mesh/2026/D016650
  2. Fluorescein-5-isothiocyanate. In: Wikidata. https://www.wikidata.org/wiki/Q59647575
  3. Fluorescein-5-isothiocyanate. In: PubChem Compound. CID 18730. https://pubchem.ncbi.nlm.nih.gov/compound/18730