Analytical, Diagnostic and Therapeutic Techniques, and Equipment

Fluorescence Multiphoton Microscopy

Fluorescence microscopy utilizing multiple low-energy photons to produce the excitation event of the fluorophore (endogenous fluorescent molecules in living tissues or FLUORESCENT DYES). Multiphoton microscopes have a simplified optical path in the emission side due to the lack of an emission pinhole, which is necessary with normal confocal microscopes. Ultimately this allows spatial isolation of the excitation event, enabling deeper imaging into optically thick tissue, while restricting photobleaching and phototoxicity to the area being imaged.

National Library of MedicineMedical Subject Headings2026

Structured Summary

Abstract

Fluorescence microscopy utilizing multiple low-energy photons to produce the excitation event of the fluorophore (endogenous fluorescent molecules in living tissues or FLUORESCENT DYES). Multiphoton microscopes have a simplified optical path in the emission side due to the lack of an emission pinhole, which is necessary with normal confocal microscopes. Ultimately this allows spatial isolation of the excitation event, enabling deeper imaging into optically thick tissue, while restricting photobleaching and phototoxicity to the area being imaged.

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Synonyms

10 entry terms
  • Fluorescence Microscopy, Multiphoton
  • Microscopy, Fluorescence, Multiphoton
  • Multiphoton Excitation Microscopy
  • Multiphoton Fluorescence Microscopy
  • Excitation Microscopies, Multiphoton
  • Excitation Microscopy, Multiphoton
  • Microscopies, Multiphoton Excitation
  • Microscopy, Multiphoton Excitation
  • Microscopy, Multiphoton Fluorescence
  • Multiphoton Excitation Microscopies

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Aspects Covered

14 allowable subheadings

Indexed with the subheadings adverse effects, classification, economics, ethics, history, instrumentation, methods, mortality, nursing, psychology, standards, statistics & numerical data, trends, veterinary.

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History Note

2003

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Previous Indexing

  • Microscopy, Confocal (1997-2002)
  • Microscopy, Fluorescence (1996-2002)

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AMA Style

References

  1. National Library of Medicine. Fluorescence Multiphoton Microscopy. Medical Subject Headings (MeSH). 2026. Unique ID D036641. http://id.nlm.nih.gov/mesh/2026/D036641
  2. Fluorescence Multiphoton Microscopy. In: Wikidata. https://www.wikidata.org/wiki/Q73040214