Analytical, Diagnostic and Therapeutic Techniques, and Equipment

Fluorescence Recovery After Photobleaching

A method used to study the lateral movement of MEMBRANE PROTEINS and LIPIDS. A small area of a cell membrane is bleached by laser light and the amount of time necessary for unbleached fluorescent marker-tagged proteins to diffuse back into the bleached site is a measurement of the cell membrane's fluidity. The diffusion coefficient of a protein or lipid in the membrane can be calculated from the data. (From Segen, Current Med Talk, 1995).

National Library of MedicineMedical Subject Headings2026

Structured Summary

Abstract

A method used to study the lateral movement of MEMBRANE PROTEINS and LIPIDS. A small area of a cell membrane is bleached by laser light and the amount of time necessary for unbleached fluorescent marker-tagged proteins to diffuse back into the bleached site is a measurement of the cell membrane's fluidity. The diffusion coefficient of a protein or lipid in the membrane can be calculated from the data. (From Segen, Current Med Talk, 1995).

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Synonyms

3 entry terms
  • FRAP (Fluorescence Recovery After Photobleaching)
  • Fluorescence Photobleaching Recovery
  • FRAPs (Fluorescence Recovery After Photobleaching)

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Aspects Covered

10 allowable subheadings

Indexed with the subheadings classification, economics, ethics, history, instrumentation, methods, standards, statistics & numerical data, trends, veterinary.

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History Note

2003

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Previous Indexing

  • Fluorescence (1976-2002)

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References

  1. National Library of Medicine. Fluorescence Recovery After Photobleaching. Medical Subject Headings (MeSH). 2026. Unique ID D036681. http://id.nlm.nih.gov/mesh/2026/D036681
  2. Fluorescence Recovery After Photobleaching. In: Wikipedia. https://en.wikipedia.org/wiki/Fluorescence_recovery_after_photobleaching
  3. Fluorescence Recovery After Photobleaching. In: Wikidata. https://www.wikidata.org/wiki/Q729923