Structured Summary
Abstract
A DNA amplification technique based upon the ligation of OLIGONUCLEOTIDE PROBES. The probes are designed to exactly match two adjacent sequences of a specific target DNA. The chain reaction is repeated in three steps in the presence of excess probe: (1) heat denaturation of double-stranded DNA, (2) annealing of probes to target DNA, and (3) joining of the probes by thermostable DNA ligase. After the reaction is repeated for 20-30 cycles the production of ligated probe is measured.
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Synonyms
5 entry terms
- Chain Reaction, Ligase
- Chain Reactions, Ligase
- Ligase Chain Reactions
- Reaction, Ligase Chain
- Reactions, Ligase Chain
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Aspects Covered
10 allowable subheadings
Indexed with the subheadings classification, economics, ethics, history, instrumentation, methods, standards, statistics & numerical data, trends, veterinary.
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History Note
2001
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Previous Indexing
- Gene Amplification (1980-2000)
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AMA Style
References
- National Library of Medicine. Ligase Chain Reaction. Medical Subject Headings (MeSH). 2026. Unique ID D022521. http://id.nlm.nih.gov/mesh/2026/D022521
- Ligase Chain Reaction. In: Wikipedia. https://en.wikipedia.org/wiki/Ligase_chain_reaction
- Ligase Chain Reaction. In: Wikidata. https://www.wikidata.org/wiki/Q1422343