Analytical, Diagnostic and Therapeutic Techniques, and Equipment

Microspectrophotometry

Analytical technique for studying substances present at enzyme concentrations in single cells, in situ, by measuring light absorption. Light from a tungsten strip lamp or xenon arc dispersed by a grating monochromator illuminates the optical system of a microscope. The absorbance of light is measured (in nanometers) by comparing the difference between the image of the sample and a reference image.

National Library of MedicineMedical Subject Headings2026

Structured Summary

Abstract

Analytical technique for studying substances present at enzyme concentrations in single cells, in situ, by measuring light absorption. Light from a tungsten strip lamp or xenon arc dispersed by a grating monochromator illuminates the optical system of a microscope. The absorbance of light is measured (in nanometers) by comparing the difference between the image of the sample and a reference image.

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Synonyms

1 entry terms
  • Microspectrophotometries

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Aspects Covered

10 allowable subheadings

Indexed with the subheadings classification, economics, ethics, history, instrumentation, methods, standards, statistics & numerical data, trends, veterinary.

MeSH Record

Indexing Annotation

a type of spectrum anal; NIM

MeSH Record

History Note

90

MeSH Record

Previous Indexing

  • Spectrophotometry (1966-1989)

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AMA Style

References

  1. National Library of Medicine. Microspectrophotometry. Medical Subject Headings (MeSH). 2026. Unique ID D015591. http://id.nlm.nih.gov/mesh/2026/D015591
  2. Microspectrophotometry. In: Wikipedia. https://en.wikipedia.org/wiki/Micro-spectrophotometry
  3. Microspectrophotometry. In: Wikidata. https://www.wikidata.org/wiki/Q12037087