Analytical, Diagnostic and Therapeutic Techniques, and Equipment

Negative Staining

The technique of washing tissue specimens with a concentrated solution of a heavy metal salt and letting it dry. The specimen will be covered with a very thin layer of the metal salt, being excluded in areas where an adsorbed macromolecule is present. The macromolecules allow electrons from the beam of an electron microscope to pass much more readily than the heavy metal; thus, a reversed or negative image of the molecule is created.

National Library of MedicineMedical Subject Headings2026

Structured Summary

Abstract

The technique of washing tissue specimens with a concentrated solution of a heavy metal salt and letting it dry. The specimen will be covered with a very thin layer of the metal salt, being excluded in areas where an adsorbed macromolecule is present. The macromolecules allow electrons from the beam of an electron microscope to pass much more readily than the heavy metal; thus, a reversed or negative image of the molecule is created.

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Synonyms

1 entry terms
  • Staining, Negative

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Aspects Covered

10 allowable subheadings

Indexed with the subheadings classification, economics, ethics, history, instrumentation, methods, standards, statistics & numerical data, trends, veterinary.

MeSH Record

History Note

92

MeSH Record

Previous Indexing

  • Staining (1966-1991)

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AMA Style

References

  1. National Library of Medicine. Negative Staining. Medical Subject Headings (MeSH). 2026. Unique ID D016624. http://id.nlm.nih.gov/mesh/2026/D016624
  2. Negative Staining. In: Wikipedia. https://en.wikipedia.org/wiki/Negative_stain
  3. Negative Staining. In: Wikidata. https://www.wikidata.org/wiki/Q3772564