Analytical, Diagnostic and Therapeutic Techniques, and Equipment

Protein Footprinting

A method for determining points of contact between interacting proteins or binding sites of proteins to nucleic acids. Protein footprinting utilizes a protein cutting reagent or protease. Protein cleavage is inhibited where the proteins, or nucleic acids and protein, contact each other. After completion of the cutting reaction, the remaining peptide fragments are analyzed by electrophoresis.

National Library of MedicineMedical Subject Headings2026

Structured Summary

Abstract

A method for determining points of contact between interacting proteins or binding sites of proteins to nucleic acids. Protein footprinting utilizes a protein cutting reagent or protease. Protein cleavage is inhibited where the proteins, or nucleic acids and protein, contact each other. After completion of the cutting reaction, the remaining peptide fragments are analyzed by electrophoresis.

MeSH Record

Classification

Broader headings

Related Concepts

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MeSH Record

Synonyms

5 entry terms
  • Footprinting, Protein
  • Footprints, Protein
  • Protein Footprints
  • Footprint, Protein
  • Protein Footprint

MeSH Record

Aspects Covered

11 allowable subheadings

Indexed with the subheadings adverse effects, classification, economics, ethics, history, instrumentation, methods, standards, statistics & numerical data, trends, veterinary.

MeSH Record

History Note

2001

MeSH Hierarchy

Tree Numbers

  1. E05.196w.700.690w
  2. E05.393.760w.705w.685.690w

AMA Style

References

  1. National Library of Medicine. Protein Footprinting. Medical Subject Headings (MeSH). 2026. Unique ID D023302. http://id.nlm.nih.gov/mesh/2026/D023302
  2. Protein Footprinting. In: Wikipedia. https://en.wikipedia.org/wiki/Protein_footprinting
  3. Protein Footprinting. In: Wikidata. https://www.wikidata.org/wiki/Q7251433