Analytical, Diagnostic and Therapeutic Techniques, and Equipment

Two-Hybrid System Techniques

Screening techniques first developed in yeast to identify genes encoding interacting proteins. Variations are used to evaluate interplay between proteins and other molecules. Two-hybrid techniques refer to analysis for protein-protein interactions, one-hybrid for DNA-protein interactions, three-hybrid interactions for RNA-protein interactions or ligand-based interactions. Reverse n-hybrid techniques refer to analysis for mutations or other small molecules that dissociate known interactions.

National Library of MedicineMedical Subject Headings2026

Structured Summary

Abstract

Screening techniques first developed in yeast to identify genes encoding interacting proteins. Variations are used to evaluate interplay between proteins and other molecules. Two-hybrid techniques refer to analysis for protein-protein interactions, one-hybrid for DNA-protein interactions, three-hybrid interactions for RNA-protein interactions or ligand-based interactions. Reverse n-hybrid techniques refer to analysis for mutations or other small molecules that dissociate known interactions.

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Synonyms

91 entry terms
  • Two-Hybrid Assay
  • Two-Hybrid Method
  • Two-Hybrid System Technics
  • Assay, Two-Hybrid
  • Assays, Two-Hybrid
  • Method, Two-Hybrid
  • Methods, Two-Hybrid
  • Technic, Two-Hybrid System
  • Technics, Two-Hybrid System
  • Technique, Two-Hybrid System
  • Techniques, Two-Hybrid System
  • Two Hybrid Assay
  • Two Hybrid Method
  • Two Hybrid System Technics
  • Two Hybrid System Techniques
  • Two-Hybrid Assays
  • Two-Hybrid Methods
  • Two-Hybrid System Technic
  • Two-Hybrid System Technique
  • One-Hybrid System Technics
  • One-Hybrid System Techniques
  • Reverse One-Hybrid System Techniques
  • Reverse Three-Hybrid System Techniques
  • Reverse Two-Hybrid System Techniques
  • Three-Hybrid System Technics
  • Three-Hybrid System Techniques
  • Tri-Hybrid System Techniques
  • Yeast One-Hybrid System Techniques
  • Yeast Three-Hybrid Assay
  • Yeast Three-Hybrid System
  • Yeast Three-Hybrid System Techniques
  • Yeast Two-Hybrid Assay
  • Yeast Two-Hybrid System
  • Yeast Two-Hybrid System Techniques
  • n-Hybrid System Techniques
  • Assay, Yeast Three-Hybrid
  • Assay, Yeast Two-Hybrid
  • Assays, Yeast Three-Hybrid
  • Assays, Yeast Two-Hybrid
  • One Hybrid System Technics
  • One Hybrid System Techniques
  • One-Hybrid System Technic
  • One-Hybrid System Technique
  • Reverse One Hybrid System Techniques
  • Reverse Three Hybrid System Techniques
  • Reverse Two Hybrid System Techniques
  • System Technique, n-Hybrid
  • System Techniques, n-Hybrid
  • System, Yeast Three-Hybrid
  • System, Yeast Two-Hybrid
  • Systems, Yeast Three-Hybrid
  • Systems, Yeast Two-Hybrid
  • Technic, One-Hybrid System
  • Technic, Three-Hybrid System
  • Technics, One-Hybrid System
  • Technics, Three-Hybrid System
  • Technique, One-Hybrid System
  • Technique, Three-Hybrid System
  • Technique, Tri-Hybrid System
  • Technique, n-Hybrid System
  • Techniques, One-Hybrid System
  • Techniques, Three-Hybrid System
  • Techniques, Tri-Hybrid System
  • Techniques, n-Hybrid System
  • Three Hybrid System Technics
  • Three Hybrid System Techniques
  • Three-Hybrid Assay, Yeast
  • Three-Hybrid Assays, Yeast
  • Three-Hybrid System Technic
  • Three-Hybrid System Technique
  • Three-Hybrid System, Yeast
  • Three-Hybrid Systems, Yeast
  • Tri Hybrid System Techniques
  • Tri-Hybrid System Technique
  • Two-Hybrid Assay, Yeast
  • Two-Hybrid Assays, Yeast
  • Two-Hybrid System, Yeast
  • Two-Hybrid Systems, Yeast
  • Yeast One Hybrid System Techniques
  • Yeast Three Hybrid Assay
  • Yeast Three Hybrid System
  • Yeast Three Hybrid System Techniques
  • Yeast Three-Hybrid Assays
  • Yeast Three-Hybrid Systems
  • Yeast Two Hybrid Assay
  • Yeast Two Hybrid System
  • Yeast Two Hybrid System Techniques
  • Yeast Two-Hybrid Assays
  • Yeast Two-Hybrid Systems
  • n Hybrid System Techniques
  • n-Hybrid System Technique

MeSH Record

Aspects Covered

13 allowable subheadings

Indexed with the subheadings adverse effects, classification, economics, ethics, history, instrumentation, mortality, nursing, psychology, standards, statistics & numerical data, trends, veterinary.

MeSH Record

History Note

2000

MeSH Hierarchy

Tree Numbers

  1. E05.393.220w.870w
  2. E05.601.690.650w
  3. E05.601.870w

AMA Style

References

  1. National Library of Medicine. Two-Hybrid System Techniques. Medical Subject Headings (MeSH). 2026. Unique ID D020798. http://id.nlm.nih.gov/mesh/2026/D020798
  2. Two-Hybrid System Techniques. In: Wikipedia. https://en.wikipedia.org/wiki/Two-hybrid_screening
  3. Two-Hybrid System Techniques. In: Wikidata. https://www.wikidata.org/wiki/Q1337844