Structured Summary
Abstract
A replica technique in which cells are frozen to a very low temperature and cracked with a knife blade to expose the interior surfaces of the cells or cell membranes. The cracked cell surfaces are then freeze-dried to expose their constituents. The surfaces are now ready for shadowing to be viewed using an electron microscope. This method differs from freeze-fracturing in that no cryoprotectant is used and, thus, allows for the sublimation of water during the freeze-drying process to etch the surfaces.
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Synonyms
1 entry terms
- Etching, Freeze
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Aspects Covered
10 allowable subheadings
Indexed with the subheadings classification, economics, ethics, history, instrumentation, methods, standards, statistics & numerical data, trends, veterinary.
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Indexing Annotation
do not confuse with FREEZE FRACTURING (wherein a cryoprotectant is used)
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History Note
73(69)
MeSH Record
Previous Indexing
- Freezing (1966-1968)
- Histological Techniques (1966-1968)
- Microscopy, Electron (1966-1968)
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AMA Style
References
- National Library of Medicine. Freeze Etching. Medical Subject Headings (MeSH). 2026. Unique ID D005613. http://id.nlm.nih.gov/mesh/2026/D005613
- Freeze Etching. In: Wikidata. https://www.wikidata.org/wiki/Q9263700