Analytical, Diagnostic and Therapeutic Techniques, and Equipment

Southern Blotting

A method (first developed by E.M. Southern) for detection of DNA that has been electrophoretically separated and immobilized by blotting on nitrocellulose or other type of paper or nylon membrane followed by hybridization with labeled NUCLEIC ACID PROBES.

National Library of MedicineMedical Subject Headings2026

Structured Summary

Abstract

A method (first developed by E.M. Southern) for detection of DNA that has been electrophoretically separated and immobilized by blotting on nitrocellulose or other type of paper or nylon membrane followed by hybridization with labeled NUCLEIC ACID PROBES.

MeSH Record

Classification

Broader headings

Related Concepts

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See Also

MeSH Record

Synonyms

3 entry terms
  • Blotting, Southern
  • Blot, Southern
  • Southern Blot

MeSH Record

Aspects Covered

10 allowable subheadings

Indexed with the subheadings classification, economics, ethics, history, instrumentation, methods, standards, statistics & numerical data, trends, veterinary.

MeSH Record

Indexing Annotation

"Southern" (developed by E.M. Southern) is capitalized in titles & translations

MeSH Record

History Note

89

MeSH Record

Previous Indexing

  • DNA or specific (1975-1988)
  • Electrophoresis, Agar Gel (1979-1988)
  • Electrophoresis, Polyacrylamide Gel (1980-1988)
  • Nucleic Acid Hybridization (1980-1988)

MeSH Hierarchy

Tree Numbers

  1. E05.196w.401w.114w
  2. E05.301.300w.087w
  3. E05.601.150w

AMA Style

References

  1. National Library of Medicine. Southern Blotting. Medical Subject Headings (MeSH). 2026. Unique ID D015139. http://id.nlm.nih.gov/mesh/2026/D015139
  2. Southern Blotting. In: Wikipedia. https://en.wikipedia.org/wiki/Southern_blot
  3. Southern Blotting. In: Wikidata. https://www.wikidata.org/wiki/Q48749