Analytical, Diagnostic and Therapeutic Techniques, and Equipment

Nucleic Acid Hybridization

Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503)

National Library of MedicineMedical Subject Headings2026

Structured Summary

Abstract

Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503)

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Synonyms

9 entry terms
  • Acid Hybridization, Nucleic
  • Acid Hybridizations, Nucleic
  • Hybridization, Nucleic Acid
  • Hybridizations, Nucleic Acid
  • Nucleic Acid Hybridizations
  • Genomic Hybridization
  • Genomic Hybridizations
  • Hybridization, Genomic
  • Hybridizations, Genomic

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Aspects Covered

7 allowable subheadings

Indexed with the subheadings drug effects, ethics, genetics, immunology, methods, physiology, radiation effects.

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Indexing Annotation

IM general only; coord NIM with DNA or RNA or specific (IM); IN SITU HYBRIDIZATION & IN SITU HYBRIDIZATION, FLUORESCENCE are available

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History Note

72(71)

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Previous Indexing

  • DNA (1966-1970)
  • RNA (1966-1970)

MeSH Hierarchy

Tree Numbers

  1. E05.393.661w
  2. G02.111.611w

MeSH Record

NLM Classification

QU 58

AMA Style

References

  1. National Library of Medicine. Nucleic Acid Hybridization. Medical Subject Headings (MeSH). 2026. Unique ID D009693. http://id.nlm.nih.gov/mesh/2026/D009693
  2. Nucleic Acid Hybridization. In: Wikipedia. https://en.wikipedia.org/wiki/Nucleic_acid_hybridization
  3. Nucleic Acid Hybridization. In: Wikidata. https://www.wikidata.org/wiki/Q8223839